Visualizing fibril growth of an artificial peptide containing two palmitoyl membrane anchors in a model raft mixture consisting of DOPC/DPPC/Chol 1:2:1. The partitioning of the peptide into the membrane induces fluid domains and causes decrease of the membrane thickness of the fluid domains (ld) of around 1 nm. Growth of fibrils begins within the first 30 min after injection of peptide solution into the AFM Tapping Mode fluid cell and fibrils are around 90-400 nm long and 1.0-4.9 nm thick. The image was taken with a MultiMode (Digital Instruments) and scan size is 4.5 µm.
Courtesy of Katrin Weise (1), Diana Olschewski (2), Christian Becker (2), Roland Winter (1)
(1) Physical Chemistry I Biophysical Chemistry, Department of Chemistry, University of Dortmund, Otto-Hahn-Str.6, 44227 Dortmund, Germany
(2) Max-Planck Institute of Molecular Physiology, Department of Physical Biochemistry, Otto-Hahn Str. 11, 44227 Dortmund, Germany
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