Fixed MRC-5 Fibroblast Cells Upper Left: Fluorescent image of cells labeled with DAPI (nucleus) and Alexa Fluor 488 (actin filaments) and viewed with wide-fluorescence using the Nikon TE2000U inverted optical microscope and a 40x objective. Lower Left: AFM deflection image, 60µm scan. Right: AFM deflection data (50% transparency) overlaid onto merged fluorescence optical image.
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